THALLIUM FLUX:
POTASSIUM CHANNEL
Concentration-dependent effects of positive (PAM) and negative (NAM) allosteric modulators of the G-protein-gated inward rectifying potassium channel (GIRK). Fluorescence measurement of thallium (Tl+) flux were obtained on PANOPTIC using the Tl+-sensitive dye, Thallos, at 1 Hz (480/40x, 535/40m).
THALLIUM FLUX:
SODIUM CHANNEL
Concentration-dependent effects of veratridine (an inhibitor of voltage-gate sodium channel inactivation) on NaV1.7-expressing HEK-293 cells. Fluorescence measurement of thallium (Tl+) flux were obtained on PANOPTIC using the Tl+-sensitive dye, Thallos, at 5 Hz (480/40x, 535/40m). Veratridine concentrations 10 uM – 10 nM in 3-fold steps.
LUMINESCENCE:
cAMP GloSensor
Concentration-dependent effects of a-MSH (an agonist of the Gs-coupled GPCR, MC4R) on HEK-293 cells expressing MC4R and Promega’s GloSensorTM cAMP-sensitive luciferase. Luminescence measurement of the agonist-evoked increase in intracellular cAMP were obtained on PANOPTIC at 1/3 Hz with a 720 nm short pass emission filter, 37 C. Data provide courtesy of Dr. Roger Cone.
CALCIUM FLUX:
MITOCHONDRIA
Concentration-dependent, ATP-stimulated increases in cytoplasmic and mitochondrial Ca2+ in HEK-293 cells loaded with Fluo-8 and Rhod-2. Dual excitation, dual emission data were obtained on PANOPTIC at 1 Hz using filter sets appropriate for Fluo-8 (470/40x, 525/30m) and Rhod-2 ( 545/30x, 593/40m).
CALCIUM FLUX:
GPCR, TRIPLE ADDITION
Concentration-dependent effects of VU0238429, a positive allosteric modulator (PAM) of the M3 muscarinic acetylcholine receptor natively expressed in HEK-293 cells. Fluorescence measurements of intracellular calcium (Ca2+) were obtained on PANOPTIC at 1 Hz (480/40x, 538/40m). VU0238429 concentations: 100 uM (red) - 0 uM (purple) in 3-fold steps. Dotted lines shoe the points or addition for: 1) VU0238429, 2) ~EC20 acetylcholine, 3) ECmax acetylcholine.
NEUROTRANSMITTER
TRANSPORT ASSAY
Concentration-dependent effects of Desipramine on HEK-293 cells expressing the norepinephrine transporter (NET) using the Molecular Devices Neurotransmitter Transporter Uptake Assay Kit. Changes in intracellular fluorescence relative to the vehicle control condition (purple trace) were measure on PANOPTIC at 1 Hz (470/40x, 525/30m), 37 C. Data provide courtesy of Dr. Paige Vinson.
MEMBRANE POTENTIAL:
MITOCHONDRIA
Concentration-dependent effects of CCCP on mitochondrial membrane potential measured using JC-10. Dual excitation, dual emission data were obtained on PANOPTIC at 1 Hz using filter sets appropriate for JC-10 monomer (470/40x, 525/30m) and JC-10 aggregate (525/40x, 593/40m).